AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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STUDIA CHEMIA - Ediţia nr.2, Tom II din 2017 | |||||||
Articol: |
HETEROLOGOUS EXPRESSION AND PURIFICATION OF RECOMBINANT PROAPOPTOTIC HUMAN PROTEIN SMAC/DIABLO WITH EGFP AS FUSION PARTNER. Autori: PÁL SALAMON, ILDIKÓ MIKLÓSSY, BEÁTA ALBERT, MÓNIKA KORODI, KATALIN NAGY, ILDIKÓ BAKOS, SZABOLCS LÁNYI, CSONGOR ORBÁN. |
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Rezumat: DOI: https://doi.org/10.24193/subbchem.2017.2.26 Published Online: 2017-06-20 Published Print: 2017-06-30 VIEW PDF: HETEROLOGOUS EXPRESSION AND PURIFICATION… New proteins as molecular targets in development of therapies are discovered every day. However, study of their interactions with other proteins or binding partners in complex cellular environments has its limits. Therefore, high-yield production of these proteins in heterologous systems is a valid necessity, while obtaining these proteins linked to suitable fluorescent markers represents a step ahead in protein-protein interaction studies and cellular or subcellular localization. In this study, we present production of human SMAC/Diablo recombinant protein with EGFP as a fusion partner. High-yield expression of the fusion protein was carried out in E. coli Rosetta™ (DE3)pLysS strain, and an acceptable purity of the protein was obtained after affinity chromatography purification and gel filtration. The obtained protein can be further used in protein-protein interaction studies, whereas our method represents a cost-effective and efficient production method for EGFP-fused proteins, applicable for a number of therapeutically important polypeptides. Keywords: SMAC/Diablo, apoptosis, heterologous expression, EGFP fusion proteins |
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